Metabolic profiling and in vitro assessment of anthelmintic fractions of Picria fel-terrae Lour.

نویسندگان

  • Rasika Kumarasingha
  • Avinash V. Karpe
  • Sarah Preston
  • Tiong-Chia Yeo
  • Diana S.L. Lim
  • Chu-Lee Tu
  • Jennii Luu
  • Kaylene J. Simpson
  • Jillian M. Shaw
  • Robin B. Gasser
  • David J. Beale
  • Paul D. Morrison
  • Enzo A. Palombo
  • Peter R. Boag
چکیده

Anthelmintic resistance is widespread in gastrointestinal nematode populations, such that there is a consistent need to search for new anthelmintics. However, the cost of screening for new compounds is high and has a very low success rate. Using the knowledge of traditional healers from Borneo Rainforests (Sarawak, Malaysia), we have previously shown that some traditional medicinal plants are a rich source of potential new anthelmintic drug candidates. In this study, Picria fel-terrae Lour. plant extract, which has previously shown promising anthelmintic activities, was fractionated via the use of a solid phase extraction cartridge and each isolated fraction was then tested on free-living nematode Caenorhabditis elegans and the parasitic nematode Haemonchus contortus. We found that a single fraction was enriched for nematocidal activity, killing ≥90% of C. elegans adults and inhibiting the motility of exsheathed L3 of H. contortus, while having minimal cytotoxic activity in mammalian cell culture. Metabolic profiling and chemometric analysis of the effective fraction indicated medium chained fatty acids and phenolic acids were highly represented.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Antioxidant and Antiproliferative Activities of an Ethylacetate Fraction of Picria Fel-Terrae Lour. Herbs

Background: Excessive production of oxygen free radicals and imbalance in the mechanisms responsible for antioxidant protection may result in the onset of many diseases including breast cancer. Objective: To evaluate antiproliferative and antioxidant activity of an ethylacetate fraction (EAF) of Picria fel-terrae Lour. Herbs in the T47D cell line. Methods: Phenolic and total flavonoid contents ...

متن کامل

Antioxidant Potential of Crude Extract and Different Fractions of Enhydra fluctuans Lour

The antioxidant potential of crude methanol extract (CE) as well as chloroform (CF), ethyl acetate (EF) and n-butanol (NF) soluble fractions of Enhydra fluctuans Lour, which is widely used in indigenous system of medicine for different purposes, were studied. The antioxidant potential of extract/different fractions were evaluated using different in vitro antioxidant models. In addition, total a...

متن کامل

Antioxidant Potential of Crude Extract and Different Fractions of Enhydra fluctuans Lour

The antioxidant potential of crude methanol extract (CE) as well as chloroform (CF), ethyl acetate (EF) and n-butanol (NF) soluble fractions of Enhydra fluctuans Lour, which is widely used in indigenous system of medicine for different purposes, were studied. The antioxidant potential of extract/different fractions were evaluated using different in vitro antioxidant models. In addition, total a...

متن کامل

Evaluation of phytochemical and pharmacological properties of seeds of Momordica charantia

Objective: The purpose of the current study was to investigate the in vivo (analgesic, antidiarrheal, neurological, and cytotoxic) and in vitro (antioxidant, antimicrobial, thrombolytic and anthelmintic) activity of different fractions of methanolic extract of Momordica charantia. Materials and Methods: The antioxidant property ...

متن کامل

Comparative study of Anthelmintic activity between aqueous extract of Areva Lanata and Rotula aquatica lour

Aerva lanata and Rotula aquatica Lour both plants widely distributed throughout India. This study was undertaken to in-vitro comparative study for anthelmintic effect of aqueous extract of Rotula aquatica Lour and Areva Lanata on adult earth worm’s pheritma posthuma, using piperazine citrate 15 mg/ml as standard drug. The study involved the determination of the time of paralysis and time taken ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2016